Bicine
瀹冩槸涓�绋叐鎬ч洟瀛愮珐娌栨恫锛屽畠鑸囪ū澶氬父瑕嬮噾灞舰鎴愬挤绲″悎鐗�锛涘洜姝�锛岀┅(w臎n)瀹氬父鏁�(sh霉)閬告搰姝ょ珐娌栨恫鏅傛噳鑰冩叜婵冨害銆傚畠鍙敤浜庤泲鐧借唱绲愭櫠鐨勮В姹烘柟妗堝拰浣滅偤绉诲嫊闄介洟瀛愪氦鎻涜壊璀滀腑鐨勭浉绶╂矕娑插拰娲楄劔娑�銆�
MES
鐢ㄤ簬鍚噾灞洟瀛愮殑婧舵恫涓�锛屽洜鐐哄畠涓嶆渻鑸囧ぇ澶氭暩(sh霉)閲戝爆褰㈡垚绲″悎鐗�銆侻ES閫氬父鐢ㄤ簬閰垫瘝锛岀窗鑿屽拰鍝轰钩鍕曠墿绱拌優鐨勭珐娌栧煿椁�(y菐ng)鍩轰腑锛屼絾婵冨害楂樹簬10 mM鏅傚皪妞嶇墿鏈夋瘨銆侻ES鍦ㄥ悇绋壊璀滃拰闆绘吵瀵﹂涓篃鏄緢濂界殑绶╂矕娑查伕鎿�锛屽洜鐐哄畠椤ず鍑鸿純浣庣殑闆㈠瓙閬风Щ鐜囧拰闆诲皫鐜�銆傚湪pH鍊肩偤5.5鑷�6.7鐨勮寖鍦嶅収(n猫i)锛屽畠鏄竴绋湁鏁堢殑绶╂矕鍔�銆�
MOPS
甯哥敤浜庣窗鑿�銆侀叺姣嶅拰鍝轰钩鍕曠墿绱拌優鐨勫煿椁�(y菐ng)鍩�銆傚畠閫氬父鐢ㄤ簬绱拌優鍩归(y菐ng)鍩�锛屼綔鐐洪浕娉充腑鐨勯亱琛岀珐娌栨恫鍜岃泲鐧借唱绱斿寲銆侻OPS琚獚鐐烘槸鐢ㄤ簬鍦ㄧ搳鑴傜硸涓垎闆NA鐨勬サ浣崇珐娌栨恫鍑濊啝锛岀敱浜庣櫦(f膩)鐢熺殑楂樺垎杈ㄧ巼鍒嗛洟鍜岀己涔忕浉浜掍綔鐢∕OPS鍜孯Nase鎶戝埗鍔慏EPC涔嬮枔鐨勫樊鐣�銆傚畠涔熻瑕栫偤鐢ㄩ�擲DS-PAGE涓殑閬嬭绶╂矕娑�銆�
MOPSO
瀹冪己涔忚垏澶у鏁�(sh霉)閲戝爆闆㈠瓙褰㈡垚绲″悎鐗╃殑鑳藉姏锛屼竴鑸敤浣滈噾灞洟瀛愭憾娑蹭腑鐨勯潪閰嶄綅绶╂矕娑层�傚畠鐢ㄤ綔姣涚窗绠￠浕娉充腑鐨勮級楂旈浕瑙ｈ唱锛屼互鍙婅胺鑳辩敇鑲藉悎鎴愰叾鐨勭祼鏅剁珐娌栧姂锛涘湪鐔掑厜鍏夎瓬娉�锛屽垎鍏夊厜搴︽硶鍜岀瓑婧淮瀹氶噺鐔辨硶涓娇鐢�锛涜垏鐗涜娓呯櫧铔嬬櫧锛圔SA锛夌殑鑲介鏋剁浉浜掍綔鐢紝闃叉BSA鐔辫畩鎬�锛涚敤浣滅珐娌栫偔閰垫瘝鎻愬彇鐗╁煿椁�(y菐ng)鍩虹殑绲勬垚鎴愬垎锛涚敤浣滄脯瀹氱敓鐗╂不鐞嗘捣娲嬪師娌瑰壇鐢�(ch菐n)鐗╃殑绶╂矕楂旂郴銆�
PIPES
瀹冨闅涗笂涓嶆憾浜庢按锛屼絾濡傛灉娓搁洟閰稿鍔燩IPES鐨勬憾瑙ｅ害鐢∟aOH灏囧叾杞夊寲鐐洪垑楣�銆傜己涔忚垏澶у鏁�(sh霉)閲戝爆闆㈠瓙褰㈡垚閲嶈绲″悎鐗╃殑鑳藉姏锛屼竴鑸敤浣滈噾灞洟瀛愭憾娑蹭腑鐨勯潪閰嶄綅绶╂矕娑�銆傝┎绶╂矕鍗�(q奴)鑳藉褰㈡垚鑷敱鍩�锛屽洜姝や笉閬╃敤浜庢哀鍖栭倓鍘熷弽鎳�銆傞洊鐒禤IPES鍙敤浣滈櫧闆㈠瓙浜ゆ彌鑹茶瓬涓殑绶╂矕娑诧紝浣嗗畠鎳夎┎鐢变簬鐩稿皪杓冨ぇ鐨勯洟瀛愬挤搴﹀拰婵冨害灏峱Ka鐨勪緷璩存��銆侾IPES琚獚鐐烘槸涓�绋サ濂界殑鍥哄畾绶╂矕娑茬敤浜庨浕瀛愰’寰彙鐨勬鐗╃窗鑳�銆�
HEPES
瀹冭兘杓冮暦鏅傞枔鎺у埗鎭掑畾鐨刾H鑼冨湇锛屼綆婵冨害鐨凥EPES灏嶇窗鑳炵劇姣掓�т綔鐢紝甯哥敤浜庣窗鑳炲煿椁�(y菐ng)锛屾瘮濡傚悇绋窗鑿屻�佺湡鑿岀殑鍩归(y菐ng)娑叉垨鐥呮瘨淇濆瓨娑蹭腑鍙互閫氶亷娣诲姞HEPES渚嗙董鎸佺窗鑳�銆佺窗鑿�銆佺梾姣掓墍闇�鐨刾H鐠�(hu谩n)澧冦�傚彟澶�锛屽畠鑳藉共鎿綝NA鍜岄檺鍒舵�у収(n猫i)鍒囬叾涔嬮枔鐨勫弽鎳�锛屼絾绋嬪害浣庝簬椤炰技鐨勫叿鏈夎純灏戝彇浠ｈ兒鍩虹殑绶╂矕娑�锛岄�欑ó缂轰箯骞叉摼鐨勭櫦(f膩)鐢熶富瑕佹槸鍥犵偤璩瓙鍖栧彅鑳虹殑绌洪枔浣嶉樆鍦℉EPES涓厑瑷辫垏DNA鐨勮矤闆昏嵎姣斿ぇ澶氭暩(sh霉)鏇村急銆�
HEPPSO
HEPPSO宸茶鐢ㄤ綔绯栧師鍚堟垚閰剁殑绲愭櫠绶╂矕娑查叾鍜岃垏HEPPSO寰╁悎鐨勯叾銆侶EPPSO灏嶆寚绀虹墿绋瓺aphniamagna骞句箮娌掓湁姣掓�э紝鍥犳琚獚鐐洪仼鍚堟瘨鐞嗗鐮旂┒銆傝┎绶╂矕鍗�(q奴)鑳藉褰㈡垚鑷敱鍩�锛屽洜姝や笉閬╃敤浜庢哀鍖栭倓鍘熷弽鎳�銆侶EPPSO褰㈡垚涓�鍊嬭垏Cu2+闆㈠瓙绲″悎锛涘洜姝�锛岀┅(w臎n)瀹氭�у父鏁�(sh霉)鍜屾績搴︽噳鐐哄湪鍚湁閵呴洟瀛愮殑婧舵恫涓娇鐢ㄨ┎绶╂矕娑叉檪瑕佽�冩叜鍒伴�欎竴榛炪�傞洊鐒禜EPPSO鑸囬妳闆㈠瓙绲愬悎锛屼絾瀹冧粛鐒舵槸閬╃敤浜庝簩杈涘彲瀵ч吀銆�
TAPS
浣滅偤DNA绡╁垎楂旂郴涓父鐢ㄧ殑绶╂矕娑查珨绯�锛屼篃鍙綔鐐篟NA妯ｅ搧鐨勭珐娌栨恫鎴愬垎锛涢仼鐢ㄤ簬钁夌稜楂旇杽灞ゅ倷妯ｇ殑闆诲瓙鍌抽仦鍜岀７閰稿寲鐮旂┒锛涘彲浣滅偤姣涚窗绠″崁(q奴)甯堕浕娉抽�茶铔嬬櫧寰噺鍒嗘瀽鐨勮儗鏅浕瑙ｈ唱锛涜兘缍寔鍙嶆噳楂旂郴鐨刾H鍊�銆傚父鐢ㄤ簬涓�浜涚敓鍖栬ê鏂疯│鍔戠洅銆丳CR瑷烘柗瑭﹀姂鐩掍互鍙奃NA/RNA鎻愬彇绛夎│鍔戠洅鐣朵腑銆�